Cristiano Ronaldo has again tested positive for COVID-19 and will miss the highly-anticipated reunion with Lionel Messi.
Juventus are billed to face Barcelona on Wednesday in a Uefa Champions League match in Turin.
Ronaldo has been self-isolating since he tested positive while on international duty with Portugal on October 13.
He returned another positive result last week, but underwent further testing in a bid to prove his fitness ahead of Juventus’ blockbuster tie against Barcelona.
Reports in Italy confirmed Ronaldo failed to test negative 24 hours ahead of kick-off, as required by UEFA in order to play.
The 35-year-old’s potential reunion with rival Messi was the standout fixture when the Champions League groups were drawn.
The pair, who have 11 Ballon d’or titles between them (Ronaldo, 5, Messi, 6), have not faced each other in the Champions League since the semi-final between Real Madrid and Barcelona in the 2011/12 season.
Ronaldo has missed three matches for Juventus since he tested positive, a victory over Dynamo Kiev in their Champions League Group G opener last week and two draws in Serie A.
He has been asymptomatic and posted regular updates on social media showing his home workouts and boasting a new buzzcut.
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